unconjugated lectins  (Vector Laboratories)

Journal: Disease Markers

Article Title: The Analysis of Sialylation, N -Glycan Branching, and Expression of O -Glycans in Seminal Plasma of Infertile Men

doi: 10.1155/2015/941871

Figure Lengend Snippet: The relative reactivity of seminal glycoproteins with lectins. The results are expressed in absorbance units (AU) as median values of relative reactivities with lectins. Seminal glycoproteins' lectin reactivities were estimated by direct lectin-ELISA and expressed in absorbance units (AU). Seminal groups: NF: normozoospermic fertile, N: normozoospermic infertile, A: asthenozoospermic, O: oligozoospermic, OA: oligoasthenozoospermic. MAA: Maackia amurensis agglutinin, SNA: Sambucus nigra agglutinin, MPL: Maclura pomifera lectin, VVL: Vicia villosa lectin, and PHA-L: Phaseolus vulgaris leucoagglutinin. For lectin specificity see . Significant differences versus * normozoospermic fertile, ** normozoospermic infertile, and *** asthenozoospermic groups. Statistical significance of the differences was accepted for a P value of less than 0.05.

Article Snippet: Expression of O -glycans was studied with biotinylated lectins: Maclura pomifera lectin, MPL and Vicia villosa lectin, VVL (Vector Laboratories Inc., Burlingame, CA, USA), detecting complete O -glycans and their forms truncated to single GalNAc (Tn antigen), respectively.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Parasite

Article Title: Effect of household bleach on the structure of the sporocyst wall of Toxoplasma gondii

doi: 10.1051/parasite/2021066

Figure Lengend Snippet: Maclura pomifera lectin-binding pattern of the wall of untreated, bleached and/or heated free Toxoplasma gondii sporocysts. BF, bright field; AF, autofluorescence; MPL-FITC, Maclura pomifera coupled to FITC. Bar represents 5 μm.

Article Snippet: For this, untreated or treated parasite suspensions were reacted with an FITC-conjugated Maclura pomifera lectin (MPL) (Vector labs) at 20 μg/mL in 100 μL PBS for 90 min at 37° C as described in [ ].

Techniques: Binding Assay

Journal: Colloids and Surfaces. B, Biointerfaces

Article Title: What interactions drive the salivary mucosal pellicle formation?

doi: 10.1016/j.colsurfb.2014.05.020

Figure Lengend Snippet: Demonstrating the presence of specific proteins in protein elutions indicating the presence in pellicle on each particle surface from each type of saliva secretion. How proteins detected is described: CBB – Coomassie staining, PAS – PAS staining, WB – Western blotting and MPA – lectin binding using Western blotting. Symbols indicate presence (+) or absence (−) in the three elutions: SDS1, SDS2, boil elution (WMS only, not all proteins tested). * indicates that detection levels were too low to determine protein binding pattern (N/A indicates that the protein is not present in the saliva).

Article Snippet: The membrane was then left to expose onto photographic film, developed and then fixed in the dark, followed by a water wash. Maclura pomifera agglutinin (MPA) lectin (Vector Laboratories Ltd., Peterborough, UK) was also used to visualise proteins containing a Galβ,1-3GalNAc group.

Techniques: Staining, Western Blot, Binding Assay, Protein Binding